| Our
durable culture plate models have no
harmful ingredients. They are safe to use in
classrooms. GENETIC TRANSFORMATION
Foreign genes can be inserted into E.coli to
produce and harvest specific proteins. The
biotechnology industry uses E.Coli extensively
to generate potentially beneficial products
such as insulin and Human Growth Hormone.
The insertion of a foreign gene into an organism
is called a genetic transformation.
MEDIA
To grow E.coli in a laboratory, nutrients they
need must be supplied in a culture medium.
The solid media used to grow E.coli, LB (Luria-Bertani)
agar is made of yeast extract and an enzymatic
digest of meat byproducts. The digest provides
a mixture of carbohydrates, amino acids,
nucleotides, salts and vitamins. Agar is
derived from seaweed, melts when heated and
forms a solid gel when cooled. It functions
to provide a solid support on which to culture
bacteria.
E.coli (Escherichia coli)
The bacterial culture plate model demonstrates
how E.coli (Escherichia coli is grown in
a laboratory. E. coli is the most thoroughly
understood cellular organism because it is
extremely convenient to study. It grows rapidly,
doesn't require a lot of care, is easy to
store, and what we learn can often be generalized
to cell systems. Plants and animals (including
humans.)
GFP and pBLUE
The jellyfish, Aequorea Victoria, glows in
the dark because it has a gene that codes
for GFP (Green Fluorescent Protein). E.coli
that has been transformed with this gene
will produce GFP and glow in the dark under
ultraviolet light. When transformed with
the gene pBLUE, E.coli will turn dark blue.
These two genes are often used in research
as visual aids. Transformed E.coli are much
easier to identify when they glow or change
color.
For more information, refer to our FAQ or
contact us at scivonmodels@yahoo.com |